![]() ![]() For clinical application, a more clinically relevant biofilm model for the specific biofilm infection in question should be developed to guide the amount of antibiotics used for local antibiofilm treatment. The results of OSTEOmycin™ products indicated that simple in vitro biofilm test could be used for initial screening of antibiofilm products. It is therefore necessary to taking these parameters into consideration when designing experiments. This study demonstrated biofilm removal efficacy was influenced by media, biofilm age and antibiotic concentration and treatment duration. aureus biofilms survived after 2 days treatment with vancomycin containing OSTEOmycin V™. aeruginosa biofilms after 1 day treatment, while few 72 h S. Treatment with tobramycin containing OSTEOmycin T™ removed 72 h and 168 h P. aeruginosa biofilms was found concentration-dependent and time-independent, however, increasing killing was indicated for 72 h P. In agreement with vancomycin being time-dependent, extension of the vancomycin exposure increased killing of S. Furthermore, young biofilms (24 h) were easier to eradicate than old biofilms (72 h). ![]() We found for both species that biofilms were more difficult to kill in TSB than in CaMHB. Two common bacteriological growth media, tryptic soy broth (TSB) and cation-adjusted Mueller Hinton broth (CaMHB), were tested. The commonly used Calgary biofilm device was used to grow 24 h and 72 h biofilms of Staphylococcus aureus and Pseudomonas aeruginosa, which were treated with time-dependent vancomycin (up to 3000 mg L − 1) and concentration-dependent tobramycin (up to 80 mg L − 1), respectively. Additionally, OSTEOmycin™, a clinically used antibiotic containing allograft bone product, was tested for antibiofilm efficacy. To demonstrate the importance of experimental parameters, we investigated the influence of biofilm growth age, antibiotic concentration and treatment duration, and growth media on biofilm eradication. This makes it difficult to compare the findings. ![]() However, the experimental parameters applied in these studies differ considerably, and often the rationale behind the experimental design are not well described. Numerous studies have reported minimum biofilm eradication concentration (MBEC) values of antibiotics for many known biofilm pathogens. Biofilm is known to be tolerant towards antibiotics and difficult to eradicate. ![]()
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